Overall, these results indicate that the initial seed drying affects the seed bacterial composition, suggesting that microbial isolation prior to seed drying is recommended to conserve these microbes. The main taxa were largely culturable and had similar relative abundance, while many, but not all, of the low-abundance taxa were also culturable. The changes in seed bacterial composition were reflected by cultured bacterial taxa obtained from the stored G. For many of the low-abundance Genera, storage at −20☌ resulted in their gradual disappearance, whereas storage at 4☌ or RT resulted in their more rapid disappearance. Subsequent storage at RT, 4, or −20☌ maintained high-abundance Genera at the majority of time points, although RT caused greater fluctuations in abundances. Undried seed was dominated by Pantoea (33.9%) and Pseudomonas (51.1%) however, following drying, the abundance of Pseudomonas declined significantly (0.9%), Pantoea increased significantly (73.6%), and four genera previously identified including Pajaroellobacter, Nesterenkonia, env.OPS_17, and Acidibacter were undetectable. max was dominated by Gammaproteobacteria under all conditions. Storage temperatures consisted of −20☌, 4☌, and room temperature (RT), with −20☌ being commonly used in seed storage vaults globally.

max bacterial microbiome was analyzed from undried seed, dried seed, and seed stored for 0, 3, 6, and 14months. In this study, soybean seeds were analyzed to characterize changes in the bacterial community composition and culturability under varying storage conditions. However, little is known about the impact of standard storage procedures, such as seed drying and cold storage on the seed bacterial community, and the ability to recover seed-associated bacteria after storage. Global seed vaults are important, as they conserve plant genetic resources for future breeding to improve crop yield and quality and to overcome biotic and abiotic stresses.

3Agriculture Victoria Research, Australian Grains Genebank, Horsham, VIC, Australia.2School of Applied Systems Biology, La Trobe University, Bundoora, VIC, Australia.1Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, VIC, Australia.Ankush Chandel 1,2 *, Ross Mann 1, Jatinder Kaur 1, Sally Norton 3, Jacqueline Edwards 1,2, German Spangenberg 1,2 and Timothy Sawbridge 1,2

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